Point Scan Confocal Microscope

P Series

Confocal Microscopy is an optical imaging technique for increasing the Optical Resolution and Contrast of a Micrograph by using a Spatial Pinhole to block out-of-focus light in image formation. Capturing multiple two-dimensional images at different depths in a specimen enables the reconstruction of three-dimensional imaging. Our SIMSCOP P Series offers a great balance between functionality, cost, and flexibility.

Key Advantage

2 channels for simultaneous two-color imaging
Higher acquisition speed,
Flexible selection of laser lines and detector type
Adaptable to SIPM, PMT(MA), PMT(GaAsP), PMT(GaAs)
Reserved channels support modular upgrades

Product Brochure

Point Scan Confocal Microscope

P Series

Confocal Microscopy is an optical imaging technique for increasing the Optical Resolution and Contrast of a Micrograph by using a Spatial Pinhole to block out-of-focus light in image formation. Capturing multiple two-dimensional images at different depths in a specimen enables the reconstruction of three-dimensional imaging. Our SIMSCOP P Series offers a great balance between functionality, cost, and flexibility.

Key Advantage

2 channels for simultaneous two-color imaging
Higher acquisition speed,
Flexible selection of laser lines and detector type
Adaptable to SIPM, PMT(MA), PMT(GaAsP), PMT(GaAs)
Reserved channels support modular upgrades

Product Brochure

Confocal Microscopy is an optical imaging technique for increasing the Optical Resolution and Contrast of a Micrograph by using a Spatial Pinhole to block out-of-focus light in image formation. Capturing multiple two-dimensional images at different depths in a specimen enables the reconstruction of three-dimensional imaging.

Our recently released “2+1” P series confocal microscope offers 2 channel imaging detector and 1 functional detection channel with several options. The ability to simultaneously collect signals from two channels can be advantageous for dynamic studies, such as tracking the movement of labeled particles or observing live cell processes. It allows you to capture multiple aspects of the sample's behavior in real-time. The presence of a functional channel allows you to capture spectral or lifetime information from the sample, making it suitable for a wide range of applications.

Key Advantage 2 channels for simultaneous two-color imaging Higher acquisition speed, Flexible selection of laser lines and detector type Adaptable to SIPM, PMT(MA), PMT(GaAsP), PMT(GaAs) Reserved channels support modular upgrades: Raman spectrometer Fluorescence spectrometer TCSPC module NIR I/II detector


	2 detector channels enable simultaneous two-color imaging for faster imaging, You can use different fluorophores or fluorescent labels with distinct emission spectra and simultaneously capture their signals. This is especially useful for studying multiple targets or molecules within a sample without the need for sequential imaging.

Flexible selection of laser lines and detector type (GaAsP, PMT/MA, PMT/SIPM): The ability to choose detector type allows you to optimize SNR for your specific experimental requirements. The combination of multiple detectors and functional information allows for quantitative analysis of fluorescence intensities, emission spectra, and fluorescence lifetime measurements. This is crucial for many quantitative biology and materials science experiments.

Parameters	SiPM	GaAsP PMT	Polyalkaline PMT	Dual-alkali (Ultra bialkali) PMT
Photosentsitive Area	6mmx6mm	Ø5 mm	Ø8 mm	Ø8 mm
Spectral Response Range (nm)	200-900	300-740	185-870	230-700
Peak Response Wavelength (nm)	420	520	400	400
Dark Current nA	900	3	1	1
Peak Wavelength Detection Efficiency	38%@420nm	45%@520nm	23.87%@400nm	40.3%@400nm
Rise Time	180ps	1ns	0.57ns	0.57ns

Advantages of SiPM detectors: Achieve higher QE and lower noise: low voltage operation, long life, wider dynamic range, insensitive to magnetic fields, suitable for high-speed high signal-to-noise ratio imaging.

Software's UI & Function

SIMSCOP - P Series Point Scan Confocal Microscope
Laser Light Source	Standard wavelength:405±5 nm; 488nm±5nm; 561nm±1nm; 640nm±5nm Output mode:single-mode polarization-maintaining fiber (TEM00) Single wavelength output power: >20mW; Power stability: <1% Spectral linewidth: <3nm TTL modulation, 1kHz Accuracy of laser power adjustment: 0.1%; Multi-wavelength AOTF power adjustment Optional wavelengths include 375nm/ 445nm/473nm/ 515nm/525nm /532nm/633nm /660nm/ 685nm/785nm/808nm
Detectors	SiPM, 250-950nm QE＞25%@420nm, dark current: 618nA Multi-alkali PMT, 300-650nm QE>30%@500nm, dark current: 10nA GaAsP PMT, 300-740nm QE>45%@520nm, dark current: 3nA
Scan Module	Scanning pixels 128x128 ~ 4096x4096 Pixel time 0.5 μs -100 μs Maximum scanning speed: up to 8fps ( 512 x 512)
XY Resolution	230nm@100x Oil objective
Image Depth	＜100um
FOV	5X: 1.44mmx1.44mm \| 10x: 0.72mmx0.72mm \| 20x: 0.36mmx0.36mm, 40x: 0.18mmx0.18mm \| 60x: 120umx120um \| 100x: 72umx72um
Filter Unit	DAPI EM 445nm/50nm, FITC EM 530nm/50nm TRITC EM 605nm/60nm, Cy5 EM 695nm/40nm
Pinhole	16 pinhole choices; Pinhole diameter range:Ø25 µm to Ø2 mm
Eyepiece	WF10X/23 wide field eyepiece; High eye point; Centering telescope
Eyepiece Tube	45°inclined,50‒75mm adjustable interpupillary distance; Adjustable diopter
Objective Converter	Converter with five-hole internal positioning ; Ball bearing for internal positioning
Stage	Manual: 240mm x 260mm fixed stage; Range of movement:135mm x 85mm Motorized: Minimum step size:50nm; Repeatability +/- 0.1 μm Maximum speed：≥100mm/s; Stage size:≥270x170mm Stroke : X:110mm,Y:75mm; Maximum load capacity >1KG (Horizontal)
Z Driver	Focusing resolution/minimum step size: 0.05μm; Repeatability: +/-0.2μm; Maximum stroke:10mm
Focusing Mechanism	Coaxial coarse/ fine adjustment with limit and locking devices, Low level coaxial focusing handwheel ; Handwheel graduations of fine adjustment:1μm
Transmitted Illumination System	Warm LED light, continuously adjustable brightness Brightness adjuster with LED rotation Condenser:72mm ultra-long working distance,NA=0.30; Equipped with a three-hole phase contrast annular plate
Epi-fluorescence Illumination System	Multi-band LED light source MG-100 6-hole fluorescence module UV(U)EX:375/30nm； DM:415；EM:460/50nm Blue(B)EX:475/30nm； DM:505；EM:530/40nm Yellow(Y)EX:540/25nm； DM:565；EM:605/55nm Red(R)EX:620/50nm； DM:655；EM:692/45nm
Upgrade module (optional)	Raman confocal spectrometer Fluorescence spectrometer TCSPC NIR I/II detector
Software Feature	Multi-color fluorescence localization processing; Z-stack data processing; Large image stitching; Image analysis; Imaging data management; 3D imaging reconstruction; Spectrum analysis

Updating, so stay tuned!

Point Scan Confocal Microscope Acquistion Atlas

High-Definition Visual Assets: Should you require high-resolution authentic images and video footage, we invite you to get in touch with us.

Complimentary Evaluation for Samples: We extend an offer of our free examination services. Should you possess related samples that require testing, we ask that you download and complete the Sample Testing Form, and forward your samples to the designated address. For further details, please do not hesitate to Contact Us.

BPAE cells, SIMSCOP P series, 60X objective lens Laser 405nm, 488nm & 561nm (Original Image)	BPAE cells, SIMSCOP P series, 60X objective lens Laser 405nm, 488nm & 561nm (Original Image)

Mouse nerve, SIMSCOP P series, 20X objective lens Detector SIPM (Original Image)	Fibrous connective tissue section, SIMSCOP P Series, 10X objective lens_1000x1000 Detector SIPM (Original Image)

Sago palm leaf, SIMSCOP P series, 10X objective lens_500x500 Detector SIPM (Original Image)	Sago palm leaf, SIMSCOP P series, 10X objective lens_800x800 Detector SIPM (Original Image)

Our multi-line CW single-mode laser(The four-in-one laser) includes four different wavelengths(405nm/488nm/532nm/640nm or 405nm/488nm/561nm/640nm), int-egrating laser diodes, laser cavities, fiber-coupled optics, laser power supplies and LD current into one unit. It is designed for laser scanning confocal microscopy system.

The four-in-one laser in a laser scanning confocal microscopy system is a special laser system used for biomedical research and clinical applications. It combines four different wavelengths of lasers to provide multiple excitation light sources. Laser scanning confocal microscopy is a high-resolution microscopy technique that acquires the three-dimensional images of cells and tissue through laser beam scanning and focusing. The four-in-one laser provides different wavelengths of lasers for the laser scanning confocal microscopy system to excite different fluorescent dyes or markers, allowing for observation and study of samples under a microscope.

Feature

Multi-wavelength Output
High Spectral Quality
Single-mode Output
High Power Stability
Adjustable Power

Application

Imaging and Localization of Cell
Fluorescence Co-expression Studies
Imaging of Neuronal Activity
Drug Screening and Evaluation
Histopathological Analysis

Multi-Line CW Single-Mode Laser Specification

Item Number	ST4LS-A	ST4LS-B	ST4LS-C
Wavelength	405/488/532/640nm	405/488/561/640nm	Based on our standard laser configurations (405/488/561/640 and 405/488/532/640), we offer customizable options for additional wavelengths, including but not limited to: UV: 375nm，VIS: 445nm, 473nm, 515nm, 525nm, 532nm, 633nm, 660nm, 685nm，NIR: 785nm, 808nm. For further customization beyond these options, please contact us for assistance
Fiber	FC/APC
	Length: 1 Meter
	Core Diameter: 4-6 Micrometers
	Customization is avaible
Output Power	＞20mW
Power Stability(rms, over 4 hours)	<1%
Laser Noise	＜4%
Spectral Linewidth	＜3nm
Laser Power Adjustment Accuracy	Multiple Wavelength AOTF Power Adjustment Accuracy:0.1%; Without AOTF: 0.5mW
Working Mode	Continuous Output, TTL Modulation, Analog Modulation for Option
Operating Temperature	10~35(℃)
Power Supply	100~240VAC
Cooling Method	Air Cooling
Lifespan	10000(Hours)

Machine Drawing

Product Parts List

Laser (1 set)

RS232 Data Cable (1)

Modulation line (1)

Power Cord (1)

Laser Specification

A:Maintenance end cover

B:Delivery outlet

C:Support

D:Attenuator 1

E:Attenuator 2

F:External signal interface

G:Laser switch

H:RS232 interface

I:Safety lock"interlock"

J:Power Socket

K:Power Indicator Light

L:Laser Indicator Light

1. What is AOTF? What are its benefits? When is it necessary to use the AOTF laser module in a confocal system?

Acousto-Optic Tunable Filters (AOTFs) are employed for precise wavelength selection and filtering. These filters allow for the accurate selection of specific wavelengths or ranges from a wide incident spectrum, superseding traditional fixed bandwidth filters. Their tunability facilitates the adjustment of filter traits such as bandwidth and centre wavelength, providing versatility for diverse optical systems. Typically, when selecting a confocal system, users need to evaluate whether their applications necessitate exceptional wavelength precision (or a constrained wavelength range), and balance the cost associated with the corresponding requirements.

AOTFs offer several advantages, including enhanced spatial resolution, improved signal-to-noise ratio, and reduced autofluorescence. However, they also have some drawbacks, such as significantly increased cost and price of the laser, and relatively low light and signal intensity when used for narrow wavelength output.

2. What are the confocal system's requirements for laser power stability and power tuning accuracy?

Power stability of 1% represents the range of energy fluctuations in laser power over a specified time unit. For instance, with a 20mW output power and stability of 1%, the power fluctuation range is 19.9 - 20.1mW.

The power tuning accuracy of 1% indicates the smallest value users can select for output power. For instance, with a 20mW output power and a power tuning accuracy of 1%, the minimum output value is 0.2mW.

Generally, as single-point and line-scan confocal systems primarily use single-mode lasers with a power requirement of 20mW, a laser energy accuracy and tuning range of 1% is adequate. For those with ample budget, a 0.1% energy tuning range could be considered, although the cost-effectiveness of such precision is relatively low.

3. How do SiPM and PMT differ in the point scan confocal system? What are the implications?

The table below delineates differences between various detectors. Currently, SIMSCOP's detection modules are compatible with both SIPM and PMT, and users can customize their configuration based on their specific budget and performance needs.

Detector	Sensitivity	Detection Speed	Cost
APD (Avalanche Photodiode)	High (amplification)	Fast	Medium-High
SPAD (Single-Photon Avalanche Diode)	Very high (single photon)	Very fast	High
PMT (Photomultiplier Tube)	Very high (single photon)	Fast	High
SiPM (Silicon Photomultiplier)	High (single photon)	Fast	Medium

4. What are the differences and characteristics of point scanning, line scanning, and turntable confocal methods?

For a comprehensive analysis, please refer to the provided article link.

5. Why do confocal microscopes offer superior resolution compared to traditional microscopes?

For a comprehensive analysis, please refer to the provided article link.

Point Scan Confocal Microscope-P2

SIMSCOP Multi Line CW Single Mode Laser 2024V1