Auto-Fluorescence Microscope--Simscop Instrument (Guangzhou) Co., Ltd

Automated Fluorescence Microscope

The Automated fluorescence microscope provides higher precision and functionality than ordinary microscopes by integrating advanced motion control and automation technologies.

It is suitable for high-end research laboratories and fields that require high-precision operations, such as nanotechnology, microscopic material research, etc.

Product Brochure

Automated Fluorescence Microscope

The Automated fluorescence microscope provides higher precision and functionality than ordinary microscopes by integrating advanced motion control and automation technologies.

It is suitable for high-end research laboratories and fields that require high-precision operations, such as nanotechnology, microscopic material research, etc.

Product Brochure

Features and Benefits

High-precision control : The Automated fluorescence microscope provides nanometer-level motion accuracy,including translation, rotation, and height adjustment .
Automated : The system supports automated sample processing and data acquisition, including autofocus, autoscan , automatic objective lens switching and image stitching .
High stability: The control system of the Automated fluorescence microscope is designed for high stability, which reduces vibration and error during movement and ensures image quality.
Real-time Control : Provides real-time motion control and feedback, allowing users to instantly adjust sample position and imaging conditions
Easy integration : The Automated fluorescence microscopy system can be seamlessly integrated with a variety of microscopes and imaging techniques (such as confocal microscopy .
Flexibility- Four upgrade options are available: Automated Raman confocal microscope, Automated point / line scanning confocal microscope, and Automated spinning disk confocal microscope.

Application Areas

Biomedical research
Materials Science
Semiconductors and microelectronics
Nanotechnology
Drug development

Lighting and Filtering Module

3-channel LED epi-illuminator with two wavelength options covering a wide range of popular fluorophores
Transmitted illuminator for brightfield and phase contrast imaging
3mm liquid light guide coupler supporting external laser and LED light engines
Motorized six filter cube turret for versatility or single filter cube core for simplicity
Filter wheel integration for additional flexibility and ultra-fast filter switching

Automated Fluorescence Module

Easy to integrate modules

Additional port for easily adding lasers or spectrometers into the infinite optical path
Simultaneous multi-channel imaging with available dual camera mount
Rapid, accurate and repeatable focus from integrated high-speed focus drive
Simplified sample loading with long travel objective lens retraction
Motorized 4-position objective changer module

Automated and high precision

Motorized stage

Type 1

50 mm up to 305 mm travel per axis
Up to 12 µm accuracy; 2 µm repeatability; 85 mm/s speed
Easily adjustable non-contact hall sensor limit switches
Designed for use with an X-MCC2 Series stepper motor controller or any 2-phase stepper motor controller

Type 2

130 x 100 mm or 250 x 100 mm travel options
1 nm resolution linear encoders provide 5 µm accuracy, 500 nm repeatability, and 50 nm minimum incremental move
Ultra quiet linear motors provide 750 mm/s top speed and are maintenance free
A microscopy platform module

Software's UI & Function

Illuminator	There are two wavelength options: Choice A - 385 nm, 473 nm, 568 nm Choice B - 385 nm , White Light (3000K) , 625 nm
Field of view	Max diameter 25mm PlanApochromat 4x - 2.7mm x 2.7mm PlanApochromat 10x - 1.20mm x 1.20mm PlanApochromat 20x - 0.67mm x 0.67mm Plan Apochromat 40x - 0.33mm x 0.33mm Plan Apochromat 60x - 220µm x 220µm Plan Apochromat 100x - 130µm x 130µm
Detector	95%@600nm Peak QE Pixel: 6.5μm x 6.5μm Pixel Size , 2048 x 2048 Resolution 13.3mm*13.3mm effective Area Noise : 0.2e- , Full-well Capacity 45ke- Compatible with water cooling and air cooling
Filter Cube Turret	Number of filter cubes: 6 Filter cube change time: 350ms Filter cube type: Zeiss Push & Click
XY Stage Comparison		Type 1	Type 2
	Drive	Stepper Motor	Linear Motors
	Minimum incremental move	< 0.45µm	< 50 nm
	XY Stage Repeatability	< 2µm	< 0.5µm
	Travel range	100 mm x 200 mm	130 mm x 100 mm
		205 mm x 205 mm	250 mm x 100 mm
		305 mm x 305 mm
	384- well scan time*	< 58S	< 46S
	96- well scan time*	< 19S	< 12S
	Lifetime	++	++++
Z- axis	Accuracy (unidirectional) 1.5um, repeatability < 0.2μm, maximum stroke 25mm , maximum speed 800mm/s
Objective Turret	4 -position objective lens motorized turret
Focusing stage	Focus Stage Encoder Resolution 1nm Focus Stage Minimum Incremental Move 20 nm Focus Stage Repeatability repeatability < 0.2µm
Filter Wheel	Adjacent Optic Switching Time 37 ms
Transparent Illumination System	Warm light LED , continuously adjustable brightness, LED knob-type brightness adjuster Condenser: ultra-long working distance 72mm , numerical aperture NA=0.30 , equipped with three-hole phase contrast ring plate
Software Features	Multicolor fluorescence localization processing, Z-stack data processing , large image stitching , image analysis , imaging data management , 3D imaging rendering

Automated upright fluorescence microscope

Automated inverted fluorescence microscope

Introduction of SIMSCOP Automated fluorescence microscope software

Automated fluorescence microscope software is an application developed for the fully automated fluorescence microscope system. The system integrates hardware components such as the motorized objective wheel , LED light , camera, and fully automated motor stage to achieve high-resolution 3D sample imaging and precise focal plane acquisition. The software allows users to perform fast, efficient, and non-invasive 3D imaging and positioning of samples, which is suitable for various research fields.

Key Features

Fluorescence imaging: By controlling the LED light source and camera, high-quality imaging is achieved to obtain the three-dimensional structural information of the sample.
Image acquisition: supports high-speed image acquisition, supports large-image stitching, and captures changes in dynamic processes.
Image processing: Provide image processing functions such as denoising, enhancement, pseudo color, etc. to optimize image quality.
Three-dimensional reconstruction: Reconstruction and visualization of three-dimensional sample structure is achieved through multi-level imaging data.
Real-time preview: allows users to observe sample imaging in real time, adjust parameters and obtain results in a timely manner.
Data storage and export: Supports storage of collected images and data, and exporting them into common image formats and data formats.

Advanced Upgrade Option- Automated Inverted Raman and Confocal Microscop

Combining the fully automated microscope system with confocal microscopy technology can achieve more accurate and feature-rich microscopic observations. Combining the advantages of these two can achieve significant improvements in many aspects.

Automated inverted Raman confocal microscope

Product Advantages

Optimized for biological users with multiple fluorescence channels
Can be connected to multi-channel laser light source or imaging camera
Users can use multiple cameras for fluorescence imaging acquisition and image integration
Single-point spectra/Raman signals can be collected while collecting fluorescence images
Users can freely add multiple modules

Application

Biology and Life Sciences
Materials Science
Graphene and carbon nanotubes
Nanomaterials
Catalyst
semiconductor
Pharmaceutical Quality Contrl

Automated inverted spinning disk confocal microscope

Product Advantages

Compatible with any upright or inverted microscope with a camera port.
With the perfect match with pinhole size and high light throughput, the SpinDisk Basic can be coupled with LED and LASER light source.
Custom-designed lenses are optimized to perform with a wide range of wavelengths from UV to near NIR to use a large variety of fluorophores

Automated inverted Point Scan Confocal Microscope

Automated inverted Line Scan Confocal Microscope

Point Scan products

Multi-channel imaging
Higher acquisition speed (8FPS)
Flexible choice of lasers and detectors
Suitable for SIPM and PMT detectors

Line Scan Products

High-speed acquisition (50fps@1024x1000 pixels )
Multicolor fluorescence ( up to 4 channels )
Deep imaging module ( maximum depth: 600μm)
High sensitivity
Large field of view (60X: 0.36mm, 40X: 0.54mm)

Combining artificial intelligence and automated fluorescence microscopy

Artificial intelligence and automated widefield fluorescence microscopy are a powerful combination. Together, these technologies form a feedback loop where AI image enhancement reveals more meaningful information from widefield images, eliminating the need for traditional super-resolution imaging systems, while automated image acquisition makes it possible to train and deploy AI-based image analysis models.

Combined advantages:

Super-resolution imaging
AI Image Enhancement
Maximizing throughput
Achieving consistency
Maximize versatility

The AI-enhanced image on the right shows enhanced detail and resolution, as well as reduced noise

SIMSCOP Automated fluorescence microscope supports near-infrared and ultraviolet imaging

Simscop 's Automated fluorescence microscope can easily support infrared and ultraviolet imaging by providing specific objective lenses and cameras upon request . It is suitable for biomedical and semiconductor microelectronics inspection and research fields.

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Left: Biomedicine Right: Semiconductors and microelectronics

Automated fluorescence microscopy for sample scanning

BPAE cell scanning. Zeiss 10x EC Epiplan, 50mm/s, analog gain 8x, illumination @ 385nm

Microfluidic droplet generator. Zeiss 5x N-Achroplan , scan time 1.5s

Example of large image stitching using Automated fluorescence microscopy

Auto-FM -20X -0.5NA-405/488/525- Multi-color fusion and large image stitching of mouse intestinal tissue embedded slice samples

the full image size is 1.8mmX1.25mm and is stitched from 56 partial images