Spinning Disk Confocal Microscope

SpinDisk Series - Basic / Standard / Advance

To guarantee each laboratory greater productivity without compromising the data quality, With this in mind, we have created the SpinDisk Series, an optimal solution for fast and gentle confocal imaging.

Data download

Spinning Disk Confocal Microscope

SpinDisk Series - Basic / Standard / Advance

To guarantee each laboratory greater productivity without compromising the data quality, With this in mind, we have created the SpinDisk Series, an optimal solution for fast and gentle confocal imaging.

Data download

SpinDisk Basic一种灵活、紧凑但功能齐全的转盘共聚焦解决方案，使高端荧光成像变得人人可及，可适用于各种应用。

正置或倒置显微镜系统方案

兼容任何带C口摄像头端口的正置或倒置显微镜

产品优势

高达 22 mm 视野的高速转盘共聚焦成像 - SpinDisk Basic 体积小巧，但具备快速的图像采集速度（15K rpm）和高灵敏度，轻松实现活细胞成像和大规模 3D 对象成像。凭借其大视野（FOV），SpinDisk Basic 可通过最小化扫描过程在单个画面中捕捉大型样本。
兼容宽场与转盘共聚焦成像
SpinDisk Basic 通过使用 LED 或激光作为光源，能够应对从基础到高难度的多种应用。
宽广的光谱范围，适用于多种成像需求。

Rat brain tissue section.,MIP of a large and multichannel acquisition,20x Dry.

CF Large viewCortical organoid. MIP of a large image.Dual channel and Z stack acquisition,60x Oil.

Cleared Mouse Brain 121um - 3D volume View

Software's UI & Function

To guarantee each laboratory greater productivity without compromising the data quality, With this in mind, we have created the SpinDisk Standard , an optimal solution for fast and gentle confocal imaging.

SpinDisk Standard combines high performance with flexibility:

Upright or inverted microscope system solutions. And compatible with any camera Head port for upright or inverted microscopes.
Due to the perfect match of pinhole size and high light flux, it can also be coupled with LED and laser light sources.
The option to freely choose the spinning disk geometry that best suits the application needs (e.g., depth imaging, fast live cell imaging).
Custom designed lenses optimized for ultraviolet to near infrared A wide range of fluorophores are used over a wide wavelength range.

SpinDisk Basic Light Path Diagram

The wider FOV up to 25mm translates into more information

collected and less tilles required to cover a large specimen.

.

The SpinDisk Basic enables researchers to routinely perform challenging live-imaging experiments for extended periods of time. The highest spinning disk rotation on the market allows to follow ultra-fast cell.		The multi-beam spinning method offers not only high-speed imaging but significantly reduced photo bleaching and phototoxicity. This gentle illumination combined with advanced optical sectioning makes the SpinDisk Basic the standard tool for 3D live cell imaging.

SpinDisk Advance is the next generation of spinning disk. It relies on the cutting-edge technology, advanced optical design and engineering solutions developed to meet the very high-end specifications required by modern fluorescence microscopy applications.

See Brighter – Enhanced Sensitivity and Image Quality

The disk optimized geometry together with the carefully designed optical layout gives contrast and image clarity, ensuring brighter images of dim samples.

See Faster – High-sectioning, Hi-speed

The capacity to image a field of view up to 25mm at the maximum disk rotation, makes the SpinDisk Advance the fastest confocal microscope on the market.

See More – Dual-camera, ultra-large field of view

It is also the first confocal unit that allows dual camera imaging of the full field of view on both cameras with homogenous illumination.

SpinDisk Advance Light Path Diagram

Day 50 human cerebral organoid showing CTIP2 - positive deep layer cortical neurons in white and pan-neuronal MAP2 marker signal in red. Nuclei were stained with DAPI(blue).

Multiple Modalities

The SpinDisk Advance is designed to be a truly enabling technology where performance is combined with the flexibility of a modular, expandable system. To achieve this, the SpinDisk Advance has three modes of use:

Left: Widefield - the motorized IN/OUT disk allows for a fluid widerfield/confocal transition without the need for realignment.

Middle: Spinning Disk Confocal -with SpinDisk Advance every upright or inverted microscope can benefit from confocal's advanced optical sectioning capacity.

Right: Super-resolution - the system is designed to be compatible with the SIM SpinDisk add-on, for a seamless evolution from confocal to super-resolution.

Largest FOV in the market

Due to the extensive field of view (FOV) encompassed by the SpinDisk Advance, the sample area is nearly twice as expansive as that of traditional imaging systems. This broader area translates into the acquisition of a higher quantity of data from each image, thereby reducing the requisite amount of sheet material for covering extensive samples. This significantly accelerates the research process. Additionally, the capacity for dual camera utilization serves to further expedite the pace of research.

Left: Brain organoid, stitched image: Unclear transcription factor TBR1 (Alexa 750 fluorophore, shown in magenta), microtubule-associated protein MAP2 (Alexa 488 fluorophore, shown in green), DAPI for DNA in blue. Plan Apochromat Lambda 60X oil.

Uniform illumination for data quantivication

Homogeneous illumination over the entire field of view is essential for quantitative imaging. The SpinDisk Advance illuminator is based on micro-lens technology which is able to turn a high-power laser from a multi-mode fiber into a uniform square collimated beam with over 90% homogenous illumination over the entire 25mm FOV. This unique feature allows data quality and data throughput to be increased avoiding artifacts and recovering information even from the periphery.

Seamless stitching of mouse kidney tissue section with Alexa 488 WGA, Alexa 568 Phalloidin

Seamless stitching of large samples

Combined with the 25mm FOV, micro lenses are essential for seamless stitching of images of large samples like tissues, organoids and full organisms. This ability allows you to:

Get reliable data without artifacts, avoiding any post-processing correction
Reduce photobleaching and increase speed by minimizing the overlap of tiles
Increase data throughput and guarantee data quality

Parameters	SpinDisk Series -Basic	SpinDisk Series - Standard	SpinDisk Series - Advance
Imaging mode	Wide field / Confocol / Fluorescence	Wide field / Confocol / Fluorescence	Wide field / Confocol / Fluorescence (Combined with SIM Basic module)
Field of view（FOV）	Largest diameter 22mm	Largest diameter 25mm Plan apochromatic objective:
		5x - 2.7mm x 2.7mm
		10x – 1.30mm x 1.30mm
		20x - 0.67mm x 0.67mm
		40x - 0.33mm x 0.33mm
		60x - 220µm x 220µm
		100x - 130µm x 130µm
Light source	Multimode laser: 1.5mm SMA \| LED: 3mm LLG		Multimode laser with SMA coupling
	LED light source life >20000 hours
	Power adjustment accuracy 1%
Spectrum range	Excitation 400-750 nm/Emission 400-850 nm
Speed of disk	15000 RPM
Scanning rate	100 – 1000 Fps
Disk geometry	50/250 slits for high throughput and real-time imaging applications 50/250µm pinholes optimized for laser light sources 60/220µm pinholes optimized for LED light sources Custom geometry and dual pattern turntables are available on request		50/250 slit helix for high throughput applications 50/250 µm pinholes for conventional imaging 50/400 µm wider spacing for depth imaging
Resolution	Lateral resolution (FWHM):230 nm (100X NA 1.45)	Lateral resolution (FWHM) : ~230 nm (high NA 1.4)	Lateral resolution (FWHM) : ~230 nm (high NA 1.4)
	Axial resolution (FWHM): 600 nm (100X NA 1.45)	Axial resolution (FWHM) : ~600 nm (high NA 1.4)	Axial resolution (FWHM) : ~600 nm (high NA 1.4)
Filter wheel	Excitation: 1 position (25mm)	Excitation : 8/4 position (25mm)	Excitation: 4 positions (25mm)
	Dichroic: 1 position (25.5 x 36mm UF1)	Dichroic:5 position (25.5 x 36mm UF1)	Dichroic: 3 position (32x44mm UF1)
	Emission: 5 positions (25mm)	Emission: 8 position (25mm)	Emission: 8 position(25mm)
			Camera splitter: 3 position
Mode switch	Manual	Electric
Camera type	C-mount camera		sCMOS Camera Compatible with CCD/EMCCD Camera
	sCMOS Camera Compatible with CCD/EMCCD Camera		Support double channels and double cameras imaging
Camera index	95%@600nm Peak QE
	6.5μm x 6.5μm Pixel Size，2048 x 2048 Resolution，13.3mm*13.3mm effective Area
	noise: 0.2e-, Full-well Capacity 45ke-
	Compatible with water cooling and air cooling
Compatible microscope	All series upright and inverted microscope
Eyepiece and eyepiece tube	WF 10X/23 flat field eyepiece with high eye point; Centering telescope, 45° tilt, pupil distance adjustment 50-75mm, viewability adjustable
Objective converter	Five-hole internal positioning converter, ball bearing internal positioning
Sample stage	Manual: fixed stage 240mm×260mm; Moving range: 135mm×85mm
	Electric: Minimum step: 50nm; Effective travel: X:114mm Y:75mm Repeated positioning accuracy: ±0.1um;
	Maximum speed: ≥100mm/s Table size ≥270x170mm Maximum load capacity: > 1KG (horizontal)
Z-axis drive	Focus resolution/minimum step size 0.002μm, repeated positioning accuracy +/-0.2μm, maximum stroke 10mm
Focusing mechanism	Coarse trimmer coaxial, equipped with limit device and locking device, low hand position coaxial focusing handwheel, trimmer handwheel grid value 1μm
Software	System control, multi-color fluorescence positioning processing, Z-stack data processing, large image stitching, image analysis, imaging data management, 3D imaging rendering
Installation conditions	Temperature 23 ± 5˚C, humidity 70% RH or lower (non-condensing)
Weight of switch mode	7.65 kg	11.0 Kg (24.3 lbs)	26.0 Kg (57 lbs)
Size	166.20 (w) x 234 (l) x 228.60 (h) mm	286.7 (W) x 405.1 (L) x 226.5 (H) mm	357.0 (W) x 606.0 (L) x 225.0 (H) mm

SpinDisk Confocal Microscopy Acquistion Atlas

High-Definition Visual Assets: Should you require high-definition authentic images and video footage, we invite you to get in touch with us.

Complimentary Evaluation for Samples: We extend an offer of our free examination services. Should you possess related samples that require testing, we ask that you download and complete the Sample Testing Form, and forward your samples to the designated address. For further details, please do not hesitate to Contact Us.

Mouse nerve 3D tomography, 20X objective lens, scanning depth ~100 microns

(Original Image)

Mouse nerve tomography 3D reconstruction, 20X objective lens, scanning depth ~100 microns

Mouse nerve 3D tomographic scanning, 60X objective lens, scanning depth ~100 microns，

(Original Image)

Mouse nerve tomography 3D reconstruction, 60X objective lens, scanning depth ~100 microns

(The picture has been processed with background noise reduction)

Zebrafish 3D tomography (Microscope system uses Olympus IX83)

Cell mitosis CETN1-GFP green, SiR-DNA blue (Microscope system uses Nikon Ti2)

BPAE-60X1.2NA-405nm-CF

BPAE-60X1.2NA-488nm-CF

BPAE-60X1.2NA-525nm-CF

BPAE-60X1.2NA-Polychromatic fusion-CF



Mouse cranial nerve, 20X08NA, 488nm laser channel, wide field (left) and rotating disk (right) effect comparison

SpinDisk Standard-20X -0.5NA-405/488/525

Multi-color fusion and large image Mosaic of mouse intestinal tissue embedded section samples - full image size 1.8mmX1.25mm composed of 56 partial images

Mouse intestinal tissue embedding section-
60X-1.2NA-405nm-WF

Mouse intestinal tissue embedding section-
60X-1.2NA-405nm-CF

Mouse intestinal tissue embedding section-
60X-1.2NA-488nm-CF

Mouse intestinal tissue embedding section-
60X-1.2NA-Polychromatic fusion-CF

BPAE cells2-100X-SpinDisk-405&488&525

Mouse cranial nerve, 20X05NA, 488nm laser channel
0.5um layer cut 100um thickness 3D reconstruction

1. What is the nature of the laser source utilized in disk-scanning confocal microscopy?

Unlike its counterparts, the point-scanning and line-scanning confocal systems, the disk-scanning confocal system primarily employs high-powered multimode lasers as its light source. These lasers necessitate a consistent power output exceeding 500mW and impose rigorous requirements for beam collimation and power stability. To meet these specific demands and strike an optimal balance between cost and performance, SIMSCOP has custom-engineered a suite of multi-channel multimode lasers dedicated to disk scanning applications.

2. How do point-scanning, line-scanning, and spin disk-scanning confocal microscopy differ, and what unique features does each exhibit?

For detailed analysis, pleaser refer to the article link.

SIMSCOP Multi-Line CW Multi-Mode Laser 2024V1