High-speed confocal imaging, in vivo imaging, ultra-high resolution!
SpinDisk Series - Basic / Standard / Advance
To guarantee each laboratory greater productivity without compromising the data quality, With this in mind, we have created the SpinDisk Series, an optimal solution for fast and gentle confocal imaging.
正置或倒置显微镜系统方案
兼容任何带C口摄像头端口的正置或倒置显微镜
产品优势
Rat brain tissue section.,MIP of a large and multichannel acquisition,20x Dry.
CF Large viewCortical organoid. MIP of a large image.Dual channel and Z stack acquisition,60x Oil.
Cleared Mouse Brain 121um - 3D volume View
To guarantee each laboratory greater productivity without compromising the data quality, With this in mind, we have created the SpinDisk Standard , an optimal solution for fast and gentle confocal imaging.
SpinDisk Standard combines high performance with flexibility:
The wider FOV up to 25mm translates into more information
collected and less tilles required to cover a large specimen.
.
SpinDisk Advance is the next generation of spinning disk. It relies on the cutting-edge technology, advanced optical design and engineering solutions developed to meet the very high-end specifications required by modern fluorescence microscopy applications.
The disk optimized geometry together with the carefully designed optical layout gives contrast and image clarity, ensuring brighter images of dim samples.
The capacity to image a field of view up to 25mm at the maximum disk rotation, makes the SpinDisk Advance the fastest confocal microscope on the market.
It is also the first confocal unit that allows dual camera imaging of the full field of view on both cameras with homogenous illumination.
SpinDisk Advance Light Path Diagram
Day 50 human cerebral organoid showing CTIP2 - positive deep layer cortical neurons in white and pan-neuronal MAP2 marker signal in red. Nuclei were stained with DAPI(blue).
Multiple Modalities
The SpinDisk Advance is designed to be a truly enabling technology where performance is combined with the flexibility of a modular, expandable system. To achieve this, the SpinDisk Advance has three modes of use:
Left: Widefield - the motorized IN/OUT disk allows for a fluid widerfield/confocal transition without the need for realignment.
Middle: Spinning Disk Confocal -with SpinDisk Advance every upright or inverted microscope can benefit from confocal's advanced optical sectioning capacity.
Right: Super-resolution - the system is designed to be compatible with the SIM SpinDisk add-on, for a seamless evolution from confocal to super-resolution.
Largest FOV in the market
Due to the extensive field of view (FOV) encompassed by the SpinDisk Advance, the sample area is nearly twice as expansive as that of traditional imaging systems. This broader area translates into the acquisition of a higher quantity of data from each image, thereby reducing the requisite amount of sheet material for covering extensive samples. This significantly accelerates the research process. Additionally, the capacity for dual camera utilization serves to further expedite the pace of research.
Left: Brain organoid, stitched image: Unclear transcription factor TBR1 (Alexa 750 fluorophore, shown in magenta), microtubule-associated protein MAP2 (Alexa 488 fluorophore, shown in green), DAPI for DNA in blue. Plan Apochromat Lambda 60X oil.
Uniform illumination for data quantivication
Homogeneous illumination over the entire field of view is essential for quantitative imaging. The SpinDisk Advance illuminator is based on micro-lens technology which is able to turn a high-power laser from a multi-mode fiber into a uniform square collimated beam with over 90% homogenous illumination over the entire 25mm FOV. This unique feature allows data quality and data throughput to be increased avoiding artifacts and recovering information even from the periphery.
Seamless stitching of mouse kidney tissue section with Alexa 488 WGA, Alexa 568 Phalloidin
Seamless stitching of large samples
Combined with the 25mm FOV, micro lenses are essential for seamless stitching of images of large samples like tissues, organoids and full organisms. This ability allows you to:
SpinDisk Confocal Microscopy Acquistion Atlas
High-Definition Visual Assets: Should you require high-definition authentic images and video footage, we invite you to get in touch with us.
Complimentary Evaluation for Samples: We extend an offer of our free examination services. Should you possess related samples that require testing, we ask that you download and complete the Sample Testing Form, and forward your samples to the designated address. For further details, please do not hesitate to Contact Us.
Mouse nerve 3D tomography, 20X objective lens, scanning depth ~100 microns
(Original Image)
Mouse nerve tomography 3D reconstruction, 20X objective lens, scanning depth ~100 microns
Mouse nerve 3D tomographic scanning, 60X objective lens, scanning depth ~100 microns，
Mouse nerve tomography 3D reconstruction, 60X objective lens, scanning depth ~100 microns
(The picture has been processed with background noise reduction)
Zebrafish 3D tomography (Microscope system uses Olympus IX83)
Cell mitosis CETN1-GFP green, SiR-DNA blue (Microscope system uses Nikon Ti2)
BPAE-60X1.2NA-405nm-CF
BPAE-60X1.2NA-488nm-CF
BPAE-60X1.2NA-525nm-CF
BPAE-60X1.2NA-Polychromatic fusion-CF
SpinDisk Standard-20X -0.5NA-405/488/525
Multi-color fusion and large image Mosaic of mouse intestinal tissue embedded section samples - full image size 1.8mmX1.25mm composed of 56 partial images
Mouse intestinal tissue embedding section-60X-1.2NA-405nm-WF
Mouse intestinal tissue embedding section-60X-1.2NA-405nm-CF
Mouse intestinal tissue embedding section-60X-1.2NA-488nm-CF
Mouse intestinal tissue embedding section-60X-1.2NA-Polychromatic fusion-CF
Mouse cranial nerve, 20X05NA, 488nm laser channel0.5um layer cut 100um thickness 3D reconstruction
1. What is the nature of the laser source utilized in disk-scanning confocal microscopy?
Unlike its counterparts, the point-scanning and line-scanning confocal systems, the disk-scanning confocal system primarily employs high-powered multimode lasers as its light source. These lasers necessitate a consistent power output exceeding 500mW and impose rigorous requirements for beam collimation and power stability. To meet these specific demands and strike an optimal balance between cost and performance, SIMSCOP has custom-engineered a suite of multi-channel multimode lasers dedicated to disk scanning applications.
2. How do point-scanning, line-scanning, and spin disk-scanning confocal microscopy differ, and what unique features does each exhibit?
For detailed analysis, pleaser refer to the article link.
SIMSCOP Multi-Line CW Multi-Mode Laser 2024V1